阅读:1656 ,发布于2023/11/15 10:34
In collaboration with University of California, Davis; UCD evaluated
microwave and conduction heating and presented the findings at the 28th
American Peptide Symposium
Abstract
Solid phase peptide synthesis (SPPS) has
become a standard approach for synthesis of peptides, especially in a
laboratory setting. Heating the reactions in SPPS could significantly reduce
the coupling and deprotection times. One of the heating methods is to use
microwave which is becoming increasingly popular because it not only
dramatically reduces the synthesis times, but also increases the
crude peptide purity [1]. However, microwave peptide synthesizers are
relatively expensive. In this study, we investigated whether SPPS using
conduction heating can achieve similar result as microwave irradiation. CSBIO
II and CEM Liberty Blue were used as heating resource of conduction and
microwave heating, respectively. Four peptides with length of 18mer, 19mer, 20mer
(Bivalirudin) and 39mer (Exenatide) were selected as examples. The peptides
were synthesized using the same synthesis protocol at 90 ºC including identical
coupling, deprotection and washing cycles. The differences between the two
approaches are the temperature of washing DMF (90 ºC vs 23 ºC for conduction
and microwave heating, respectively) and overall synthesis cycle time (17 min
vs 13 min in conduction and microwave heating, respectively). Both conduction
and microwave heating generated comparable results with crude purity of 52.0%
vs 51.7%, 49.0% vs 57.3%, 62.8% vs 57.1%, 37.0% vs 30.5% for 18mer, 19mer,
20mer and 39mer, respectively. One of the advantages of conduction heating is
the uniformly and consistently delivered temperature during the synthesis which
could minimize racemization and side reactions caused by spikes and hotspots
typically associated with microwave heating. In addition, conduction heating is
also a more cost-efficient heating method when compared to expensive microwave
heating technology.
Download the full poster here Download Link
About CSBio: For over 30 years, CSBio, a leading peptide and peptide
synthesizer manufacturing company located in Silicon Valley, California, has
been providing cGMP peptides and automated peptide synthesizers to the global
pharmaceutical community. CSBio’s peptide products and peptide synthesizers can
be found in production laboratories, universities, and pharmaceutical companies
worldwide.
Interested in any of our peptide
synthesizers? Get in touch
with us.
|