Custom Manufactured Antibody

antibody

Polyclonal Antibody
Monoclonal Antibody
Optional Antibody

Custom Manufactured Antibody

CSBio (Shanghai)Ltd is able to offer high quality and reliable custom manufactured antibody to every domestic and international customers.Armed with experienced antibody manufacturing staffs,we guaranteed the best quality products will be offered to our customers.Through weekly communication and feedback,our customer will be able to get hold 1st hand information on their projects.We also guaranteed the best after sales service to all our customers.Please do not hesitate to contact us at info@csbiochina.com,Tel:+8621-50781655,Fax:+8621-50781656

Polyclonal Antibody Projects

Monoclonal Antibody Projects

Conjugation Services

Phosphospecific Antibody Projects

AntibodyPurification and Labeling

Polyclonal Antibody Project
Project options	Leadtime	Services	Shipping items
Cat#X10010 Polyclonal Project(From protein to antisera)	10-12 weeks	protein checked by SDS-PAGE	2ml pre-immune sera
		immunization and sera collection	50ml antisera
		ELISA identification	ELISA result
			project report
Cat#X10020 Polyclonal Project (From protein to antigen purified antibodies)	10-12 weeks	protein checked by SDS-PAGE	2ml pre-immune sera
		immunization and sera collection	5mg purified antibodies
		ELISA identification	ELISA result
		antigen affinity purification	project report
Cat#X10030 Polyclonal Project (From peptide design to antisera)	12-15 weeks	peptide design and synthesis	2 mg peptides
		peptide conjugation	2ml pre-immune sera
		immunization and sera collection	50ml antisera
		ELISA identification	ELISA result
			project report
Cat#X10040 Polyclonal Project (From peptide design to antigen purified antibodies)	12-15 weeks	peptide design and synthesis(12-22aa,85%)	2mg peptides
		peptide conjugation	2ml pre-immune sera
		immunization and sera collection	5mg purified antibodies
		ELISA identification	ELISA result
		antigen affinity purification	project report
Cat#X10050 Polyclonal Project (From plasmid to antisera)	12-15 weeks	protein expression and purification	1mg protein
		immunization and sera collection	2ml pre-immune sera
		ELISA identification	50ml antisera
			ELISA result
			project report
Cat#X10060 Polyclonal Project (From plasmid to antigen purified antibodies)	12-15 weeks	protein expression and purification	1mg protein
		immunization and sera collection	2ml pre-immune sera
		ELISA identification	5mg purified antibodies
		antigen affinity purification	ELISA result
			project report
Cat#X10070 Polyclonal Project (From cDNA to antisera)	14-16 weeks	gene amplification	recombinant vector
		vector construction	1mg protein
		protein expression and purification	2ml pre-immune sera
		immunization and sera collection	50ml antisera
		ELISA identification	ELISA result
			project report
Cat#X10080 Polyclonal Project (From cDNA to antigen purified antibodies)	14-16 weeks	gene amplification	recombinant vector
		vector construction	1mg protein
		protein expression and purification	2ml pre-immune sera
		immunization and sera collection	5mg purified antibodies
		ELISA identification	ELISA result
		antigen affinity purification	project report
Cat#X10090 Polyclonal Project (Phospho- specific polyclonal antibodies)	13-16 weeks	peptide design and synthesis(12-22aa,85%)	2 mg peptides (unmodified & phospho)
		peptide conjugation	2ml pre-immune sera
		immunization and sera collection	5mg phospho-specific antibodies
		ELISA identification	ELISA result
		antigen affinity purification	project report
Cat#X10100 Polyclonal Project(Western guaranteed polyclonal antibodies)	15-18 weeks	peptide design and synthesis(12-22aa,85%)	2mg peptides
		peptide conjugation	2ml pre-immune sera
		immunization and sera collection	5mg purified antibodies
		ELISA & Western Blot identification	ELISA & Western Blot results
		antigen affinity purification	project report
Cat#X10110 Polyclonal Project (Western and IHC guaranteed polyclonal antibodies)	15-18 weeks	peptide design and synthesis(12-22aa,85%)	2 mg peptides
		peptide conjugation	2ml pre-immune sera
		immunization and sera collection	5mg purified antibodies
		ELISA identification	ELISA result
		Western Blot & IHC identification	Western Blot & IHC results
		antigen affinity purification	project report

Monoclonal Antibody Project
Project options	Leadtime	Services	Shipping items
Cat#X20010 Monoclonal Project (From protein to ascites purification)	18-24 weeks	protein checked by SDS-PAGE	50ul pre-immune sera (1:10dilution)
		immunization and sera collection	<5 clones
		ELISA identification	5mg purified antibodies (2 clones)
		fusion and hybridoma development	ELISA result
		isotype determination	isotype determination result
		ascites production and purification (2 clones)	project report
Cat#X20020 Monoclonal Project(From peptide design to ascites purification)	20-26 weeks	peptide design and synthesis(12-22aa,85%)	2 mg peptides
		peptide conjugation	50ul pre-immune sera (1:10dilution)
		immunization and sera collection	<5 clones
		ELISA identification	5mg purified antibodies (2 clones)
		fusion and hybridoma development	ELISA result
		isotype determination	isotype determination result
		ascites production and purification (2 clones)	project report
Cat#X20030 Monoclonal Project (From plasmid to ascites purification)	20-26 weeks	protein expression and purification	1mg protein
		immunization and sera collection	50ul pre-immune sera (1:10dilution)
		ELISA identification	<5 clones
		fusion and hybridoma development	5mg purified antibodies (2 clones)
		isotype determination	ELISA result
		ascites production and purification (2 clones)	isotype determination result
			project report
Cat#X20040 Monoclonal Project(From cDNA to ascites purification)	22-26 weeks	gene amplification	recombinant vector
		vector construction	1mg protein
		protein expression and purification	50ul pre-immune sera (1:10dilution)
		immunization and sera collection	<5 clones
		ELISA identification	5mg purified antibodies (2 clones)
		fusion and hybridoma development	ELISA result
		isotype determination	isotype determination result
		ascites production and purification (2 clones)	project report
Cat#X20050 Monoclonal Project (Phospho- specific monoclonal antibodies)	21-26 weeks	peptide design and synthesis(12-22aa,85%)	2 mg peptides (unmodified & phospho)
		peptide conjugation	50ul pre-immune sera (1:10dilution)
		immunization and sera collection	<5 clones
		ELISA identification	5mg purified antibodies (2 clones)
		fusion and hybridoma development	ELISA result
		isotype determination	isotype determination result
		ascites production and purification (2 clones)	project report
Cat#X20060 Monoclonal Project (Western guaranteed monoclonal antibodies)	20-26 weeks	peptide design and synthesis(12-22aa,85%)	2 mg peptides
		peptide conjugation	50ul pre-immune sera (1:10dilution)
		immunization and sera collection	<5 clones
		ELISA & Western Blot identification	5mg purified antibodies (2 clones)
		fusion and hybridoma development	ELISA & Western Blot results
		isotype determination	isotype determination result
		ascites production and purification (2 clones)	project report

Optional Antibody Projects
Custom Manufactured Peptide Service
Service	Leadtime
Peptide design	1-2 days
Peptide synthesis	2 weeks
Peptide conjugation(KLH/BSA/OVA,≤5mg)	2-3 days
Protein service
Service Items	Time
Gene synthesis	3-4 weeks
Gene amplification(Template provided by customer,≤1000bp)	2-3 weeks
Vector construction	2-3 weeks
recombinant selection	1 week
protein expression and purification	2-3 weeks
Polyclonal antibody service
Service	Leadtime
Protein checked by SDS-PAGE	2-3 days
Pre-immune sera collection	1 day
Immunization & sera collection (2 rabbits)	10 weeks
Immunization & sera collection (2 rabbits, Fast)	6-8 weeks
ELISA identification	2-3 days
Prescreen (ELISA)	2-3 days
Prescreen (Western Blot)	1 week
Prescreen (Dot Blot)	1 week
Additional rabbit (1rabbit)	6-10 weeks
Animal maintenance (1rabbit)	1 week
Additional bleed(10ml, 1rabbit)	1 week
Extension boost and bleed(10ml, 1rabbit)	1week
Immunization & sera collection (1 goat)	20 weeks
Immunization & sera collection (2 rats)	10 weeks
Immunization & sera collection (4 mice)	10 weeks
Immunization & sera collection (2 guinea pigs)	11 weeks
Monoclonal antibody service
Service	Leadtime
Protein checked by SDS-PAGE	2-3 days
Pre-immune sera collection	1 day
Immunization & sera collection(4 mice)	6-10 weeks
ELISA identification	2-3 days
Fusion and hybridoma development	10-16 weeks
Isotype determination	1 week
Ascites production (3 mice)	3-4 weeks
Roller bottle cell culture (1L)	5-7 weeks
Prescreen(ELISA)	2-3 days
Prescreen(Western Blot)	1 week
Prescreen(Dot Blot)	1 week
Clone maintenance(1 clone)	1 week
Clone storage(1 clone)	1week
Antibody purification service
Service	Leadtime
Saturated ammonium sulfate precipitation (≤60ml)	3-5days
Protein A/G affinity purification (≤60ml)	3-5 days
Antigen affinity column preparation	2-3 days
Antigen affinity purification (≤60ml)	3-5 days
Antibody identification service
Service	Leadtime
ELISA identification (1-10 samples)	2-3 days
Cell extraction (1 sample)	2-3 days
Tissue extraction (1 sample)	2-3 days
Protein concentration assay (1 sample)	2-3 days
Western Blot identification (1-8 samples)	1 week
Dot Blot identification (1-10 samples)	2-3 days
IHC identification (section provided by customer,1 photo)	1 week
Antibody labeling service
Service	Leadtime
FTIC	1-2 weeks
HRP labeling (≤5mg)	1-2 weeks
Biotin labeling (≤5mg)	1-2 weeks



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